Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification

Main Article Content

Oliver Stevanović
Dubravka Milanov
Milan Đilas
Nevenka Aleksić

Abstract

Highly mucoid Streptococcus uberis were isolated on blood agar from five milk samples originating from two dairy cow farms. All the isolates were CAMP test and esculin hydrolysis negative. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) following prior extraction with 70% formic acid was used for identification to the species level. Given that it is plausible that such strains could be isolated in the future from dairy cows by laboratories in Serbia and the Balkans, we consider this case report to be a useful instruction for veterinary bacteriology laboratories.

Downloads

Download data is not yet available.

Article Details

How to Cite
Stevanović, O., Milanov, D., Đilas, M., & Aleksić, N. (2022). Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification. Veterinarski Glasnik, 76(1). https://doi.org/10.2298/VETGL210929003S
Section
Case Report

References

Bradley A. J. 2002. Bovine mastitis: An evolving disease. The Veterinary Journal, 164: 116-128.

EUCAST, 2021. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0, 2021. http://www.eucast.org

Fortin M., Messier S., Julie Pare J., Higgins R. 2003. Identification of catalase-negative, non-beta-hemolytic, Gram-positive cocci isolated from milk samples. Journal of Clinical Microbiology, 41:106-109. DOI: 10.1128/JCM.41.1.106–109.

Günther J., Czabanska A., Bauer I., Leigh J. A., Otto Holst O., HansMartin Seyfert H-M. 2016. Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages. Veterinary Research 47:13. DOI 10.1186/s13567-015-0287-8.

Khan I. U., Hassan A. A., Abdulmawjood A., Lämmler C., Wolter W., Zschöck M. 2003. Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods. Journal of Veterinary Science, 4:213–223. PMID: 14685025.

Kromker V., Reinecke F., Paduch J. H., Grabowski N. 2014. Bovine Streptococcus uberis intramammary infections and mastitis. Clinical Microbiology, 3:157. DOI:10.4172/2327-5073.1000157.

Lämmler C. 1991. Biochemical and serological properties of Streptococcus uberis. ZentralblattfürVeterinärmedizinReihe B, 38:737-742.DOI: 10.1111/j.1439 0450.1991.tb00937.x.

Milanov, D. S., Đilas, M. D., Babić, J. M., Prunić, B. Z., & Velhner, M. J. 2018. MALDI-TOF MS identification of citrobacter youngae isolated from food. Food and Feed Research, 45(2), 107-112. https://doi.org/10.5937/FFR1802107M

Misra B. and Marshall R. T. 1971. Streptococcus uberis of bovine mastitis origin: isolation, characterization, and serology of two mucoid strains. Journal of Dairy Science, 55:194-199. DOI: 10.3168/jds.S0022-0302(72)85458-4

Odierno L., Calvinho L., Traverssa P., Lasagno M., Bogni C., Reinoso E. 2006. Conventional identification of Streptococcus uberis isolated from bovine mastitis in Argentinean dairy herds. Journal of Dairy Science, 89:3886-3890. DOI: 10.3168/jds.S0022-0302(06)72431-6. PMID: 16960064.

Raemy A., Meylan M., Casati S., Gaia V., Berchtold B., Boss R, Wyder A., Graber H.U. 2013. Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections. Acta Veterinaria Scandinavica, 55:53. http://www.actavetscand.com/content/55/1/53

Quin P. J., Markey B. K., Leonard F. C., FitzPatrick E. S., Fanning S., Hartigan P. J. 2013. The streptococci and related cocci, Section 2: Bacteriology, In: Veterinary Microbiology and Microbial Disease, 2nd Edition, Wiley-Blackwell, Hoboken, NJ, pp. 127-136.

Van Horn K., Woodward Z., Fernandez J. MALDI-TOF Identification of bacteria using 70% Formic acid on-plate direct extraction. Approached on 30 August 2021 at https://www.msacl.org/2013_posters/201302061445_69718_poster_Van%20Horn.pdf

Most read articles by the same author(s)