Microbiological characteristics of fish reared in purified wastewater from an abattoir
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Abstract
Wastewater from abattoirs in some countries is disposed of into water bodies without adequate removal of contaminants. Therefore, the use of wastewater in fish production could pose a serious risk for humans, fish and other aquatic organisms due to possible transfer of pathogenic bacteria in aquatic culture environments. The aims of the present study were to assess the levels of individual microorganisms in different tissues of common carp and to determine any correlation with the season of sampling and the type of analysed sample in common carp reared in an integrated production system that used purified water from an abattoir. A fish pond was filled mostly with purified water from an abattoir, but also partly with well water. Carp fingerlings were stocked in the earthen fishpond in March and reared in ambient conditions. Fish were collected in the spring and autumn of the following year and the microbiological quality was assessed. Carp fillets with skin, gills and digestive tract samples were collected individually under aseptic conditions. All analyses were performed according to standard procedures. The levels of all the examined bacteria in the fish were under prescribed hygiene norms. Also, Listeria spp., sulphite-reducing clostridia and Staphylococcus aureus were not found in the samples. Furthermore, pathogenic bacteria, Salmonella and Listeria monocytogenes were not isolated from the samples. The hygienic quality of the fish produced in purified waste water from an abattoir was acceptable, and the common carp meat was safe for human consumption.
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References
Andrews W. H, Wilson C. R., Poelma P. L., Romero A. 1977. Bacteriological survey of the channel catfish (Ictalurus punctatus) at the retail level. J. Food Sci. 42:359-363.
Dang S. T. T., Dalsgaard A. 2012. Escherichia coli contamination of fish raised in integrated pig-fish aquaculture systems in Vietnam. J. Food Prot. 75:1317-1319.
El-Shafai S.A., Gijzen H. J., Nasr F. A., El-Gohary F. A. 2004. Microbial quality of tilapia reared in fecal-contaminated ponds. Environ. Res. 95:231-238.
Emikpe B. O., Adebisi T., Adedeji O. B. 2011. Bacteria load on the skin and stomach of Clarias gariepinus and Oreochromis niloticus from Ibadan, South West Nigeria: Public health implications. J. Microbiol. Biotechnol. Res 1:52-59.
Fafioye O. 2011. Preliminary studies on water characteristics and bacterial population in high yield Kajola fish ponds. J. Agric. Ext. Rural Dev 3:68-71.
Feldhusen F. 2000. The role of seafood in bacterial foodborne diseases. Microb Infect 2:1651-1660.
Fernandes C. F., Flick G. J., Silva J. L., McCaskey T. A. 1997. Influence of processing schemes on indicative bacteria and quality of fresh aquacultured catfish fillets. J. Food Prot. 60:54-58.
Harnisz M., Tucholski S. 2010. Microbial quality of common carp and pikeperch fingerlings cultured in a pond fed with treated wastewater. Ecological Engineering, 36:466-470. https://doi.org/10.1016/j.ecoleng.2009.11.015.
Hassan R., Tecle S., Adcock B., Kellis M., Weiss J., Saupe A., Sorenson A., Klos R., Blankenship J., Blessington T., Whitlock L., Carleton H. A., Concepción-Acevedo J., Tolar B., Wise M., Neil K. P. 2018. Multistate outbreak of Salmonella Paratyphi B variant L(+) tartrate(+) and Salmonella Weltevreden infections linked to imported frozen raw tuna – United States, March-July 2015. Epidemiology and Infection, 146:1461-1467. doi:10.1017/S0950268818001462.
Hernández M. D., López M. B., Álvarez A., Ferrandini E., García B. G., Garrido M. D. 2009. Sensory, physical, chemical and microbiological changes in aquacultured meagre (Argyrosomus regius) fillets during ice storage. Food Chemistry, 114:237-245. https://doi.org/10.1016/j.foodchem.2008.09.045.
International Commission on Microbiological Specifications for Foods. 1986. Sampling plans for fish and shellfish. In: The Intl. Commission on Microbiological Specifications for Foods of the Intl. Union of Biological Societies Microorganisms in Foods, Editor G.S.A.B.Stewart. Sampling for microbiological analysis: principles and specific applications. 2nd ed. Oxford: Blackwell Scientific Publications. pp 181–96.
ISO 11290-1:2017. Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
ISO 11290-2:2017. Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 2: Enumeration method
ISO 6579-1:2017. Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp.
Kay D., Crowther J., Stapleton C. M., Wyer M. D., Fewtrell L., Anthony S., Bradford M., Edwards A., Francis C. A., Hopkins M., Kay C., McDonald A. T., Watkins J. 2008. Faecal indicator organism concentrations and catchment export coefficients in the UK. Water Research, 42:2649-2661. https://doi.org/10.1016/j.watres.2008.01.017.
Ljubojević D., Pelić M., Đorđević V., Milojević L., Ćirković M. 2016. Bacterial hazards in fish meat: The aetiologic agents of foodborne diseases. Meat Technology, 57:31-42.
Njoku O. E., Agwa O. K., Ibiene A. A. 2015. An investigation of the microbiological and physicochemical profile of some fish pond water within the Niger Delta region of Nigeria. African Journal of Food Science, 9:155-162.
Ogbondeminu F. S. 1993. The occurrence and distribution of enteric bacteria in fish and water of tropical ponds in Nigeria. Journal of Aquaculture in the Tropics, 8:61-66.
Ogbondeminu F. S., Okaeme A. N. 1989. Comparative analysis of bacterial flora associated with water and fish in manured pond. Bioscience Research Communications, 1:103-108.
Pelić M., Kartalović B., Živkov Baloš M., Mirilović M., Đorđević M., Teodorović V., Ćirković M., Ljubojević Pelić D. 2020. Health Risks associated with residual pesticide levels in fish reared in purified wastewater from slaughterhouse. Journal of the Hellenic Veterinary Medical Society, 71:1991-1996. https://doi.org/10.12681/jhvms.22941.
Rizzo L., Manaia C., Merlin C., Schwartz T., Dagot C., Ploy M. C., Michael I., Fatta-Kassinos D. 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: A review. Science of The Total Environment, 447:345-360. https://doi.org/10.1016/j.scitotenv.2013.01.032.
Sanjee S. A., Karim M. 2016. Microbiological quality assessment of frozen fish and fish processing materials from Bangladesh. International Journal of Food Science, Article ID 8605689:1-6. https://doi.org/10.1155/2016/8605689.
Sapkota A., Sapkota A. R., Kucharski M., Burke J., McKenzie S., Walker P., Lawrence R. 2008. Aquaculture practices and potential human health risks: Current knowledge and future priorities. Environment International, 34:1215-1226. https://doi.org/10.1016/j.envint.2008.04.009.
Scallan E., Griffin P. M., Angulo F. J., Tauxe R. V., Hoekstra R. M. 2011. Foodborne illness acquired in the United States—Unspecified agents. Emerging Infectious Diseases, 17: 16-22. doi: 10.3201/eid1701.091101p2.
Sidhu J. P., Toze S. G. 2009. Human pathogens and their indicators in biosolids: a literature review. Environment International, 35:187-201. https://doi.org/10.1016/j.envint.2008.07.006.
Slabbert J. L., Morgan W. S. G., Wood A. 1989. Microbiological aspects of fish cultured in wastewaters—the South African experience. Water Science and Technology, 21:307-310. https://doi.org/10.2166/wst.1989.0125.
SRPS EN ISO 21528-2:2017. Mikrobiologija lanca hrane – Horizontalna metoda za otkrivanje i određivanje broja Enterobacteriaceae – Deo 2: Tehnika brojanja kolonija [Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count technique (ISO 21528-2:2017, Corrected version 2018-06-01)].
SRPS EN ISO 4833-1:2014. Mikrobiologija lanca hrane — Horizontalna metoda za određivanje broja mikroorganizama — Deo 1: Brojanje kolonija na 30 °C tehnikom nalivanja ploče [Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique, (ISO 4833-1:2013)].
SRPS EN ISO 6888-1:2009. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird-Parker agar medium (ISO 6888-1:1999)
SRPS ISO 16649-2:2008. Mikrobiologija hrane i hrane za životinje - Horizontalna metoda za određivanje broja ß-glukuronidaza pozitivne Escherichia coli - Deo 2: Tehnika brojanja kolonija na 44 C pomoću 5-bromo-4-hloro-3-indolil ß-D-glukuronida [Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide]
SRPS ISO 4832:2014. Mikrobiologija hrane i hrane za životinje — Horizontalna metoda za određivanje broja koliforma — Tehnika brojanja kolonija [Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms — Colony-count technique]
Sterniša M., Mraz J., Možina S. S. 2016. Microbiological aspects of common carp (Cyprinus carpio) and its processing-relevance for final product quality: a review. Aquaculture International, 24:1569-1590. https://doi.org/10.1007/s10499-016-0051-8.
Surendran P. K., Joseph J., Shenoy A. V., Perigreen P. A., Iyer K. M., Gopakumar K. 1989. Studies of spoilage of commercially important tropical fishes under iced storage. Fisheries Research, 7:1-9. https://doi.org/10.1016/0165-7836(89)90002-7.
Surendraraj A., Farvin K. S., Yathavamoorthi R., Thampuran N. 2009. Enteric bacteria associated with farmed freshwater fish and its culture environment in Kerala, India. Research Journal of Microbiology, 4: 334-344.
Swartz M. N. 2002. Human diseases caused by foodborne pathogens of animal origin. Clinical Infectious Diseases, 34:111-122. https://doi.org/10.1086/340248.
Thi Phong Lan N., Dalsgaard A., Cam P. D., Mara D. 2007. Microbiological quality of fish grown in wastewater-fed and non-wastewater-fed fishponds in Hanoi, Vietnam: influence of hygiene practices in local retail markets. Journal of Water and Health, 5: 209-218.
Trust T. J, Sparrow R. A. H. 1974. The bacterial flora in the alimentary tract of freshwater salmonid fishes. Canadian Journal of Microbiology, 20:1219-1228. https://doi.org/10.1139/m74-188.
Zmyslowska I., Harnisz M., Lewandowska D. 2001. Sanitary and bacteriological evaluation of water quality during cage culture of wels (Silurus glanis L.) in cooling water. Fisheries & Aquatic Life, 9:191-199.
Zmyslowska I., Guziur J., Wozniak M., Harnisz M. 2002. Microbiological studies of carp (Cyprinus carpio L.) fingerlings wintered in cooling waters. Fisheries & Aquatic Life, 10:73-84.
Zmyslowska I., Kolman R., Krause J. 2003. Bacteriological evaluation of water, feed and sturgeon (Acipenser baeri Brandt) fry quality during intensive rearing in cooling water. Fisheries & Aquatic Life. 11:91-98.