Feasibility and echocardiographic characteristics of a three-minute stage dobutamine stress test in healthy dogs

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Ljubica Spasojević Kosić
Dragiša R. Trailović


Introduction. Dobutamine stress test (DST) is used as a method of functional cardiac examination in human medicine due to dobutamine’s effect, which is similar to moderate physical exercise. The aim of this study was to assess the feasibility and echocardiographic characteristics of a conventional DST in dogs.

Materials and Methods. Ten clinically healthy German Shepherd dogs were submitted to a conventional 3-minute stage DST protocol (the initial dose 7.5 μg/kg/min was increased at 3-minute intervals by 5 μg/kg/min until a maximum dosage of 42.5 μg/kg/ min; the maximum DST duration was 24 minutes), which is used in human medicine. Dobutamine action was monitored by noninvasive diagnostic methods (simultaneously lead II electrocardiography and transthoracic echocardiography). Left ventricular echocardiographic parameters were measured at baseline and at the peak of DST.

Results and Conclusions. The duration of conventional DST in dogs was 19.4±4.06 minutes. During the DST, sinus tachycardia was registered with no abnormalities of cardiac rhythm. The adverse effects registered in this study were excitement, panting and weakness. Interventricular septum thickness in systole (P=0.0093) and diastole (P=0.0080) increased significantly at the peak of DST. The left ventricular endsystolic dimension (P=0.0077) and the diastolic wall stress index (P=0.0125) decreased significantly at the peak of DST. A 3-minute stage with increased doses of dobutamine is a feasible method for the DST in dogs. The registered changes could be explained by the dobutamine effects through β1 adrenergic receptors. This pharmacological stress test induces significant changes in left ventricular echocardiographic parameters in healthy adult dogs. Dogs exhibit transient signs like sinus tachycardia, excitement, panting and weakness during DST. Additional research on DST should evaluate the diagnostic value of this protocol in dogs suffering from cardiac disease.


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Spasojević Kosić, L., & Trailović, D. R. (2018). Feasibility and echocardiographic characteristics of a three-minute stage dobutamine stress test in healthy dogs. Veterinarski Glasnik, 72(2), 90-100. https://doi.org/10.2298/ETGL180324010S
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Aurigemma, G.P., Gaasch, W.H. 2007. Quantitative evaluation of left ventricular structure, wall stress and systolic function. In The practice of clinical echocardiography. Ed. Otto, C.M. Saunders Philadelphia, pp. 187.

Cnota, J.F., Mays, W.A., Knecht, S.K., Kopser, S., Michelfelder, E.C., Knilans, T.K., Claytor, R.P., Kimball, T.R. 2003. Cardiovascular physiology during supine cycle ergometry and dobutamine stress. Medicine and Science in Sports and Exercise, 35:1503-1510. DOI: 10.1249/01.MSS.0000084436.15808.52

Coma-Canella, I. 1991. Changes in plasma potassium during the dobutamine stress test. International Journal of Cardiology, 33:55-59. DOI: https://doi.org/10.1016/0167- 5273(91)90152-F

Frye, M.A., Bright, J.M., Dargatz, D.A., Fettman, M.J., Frisbie, D.D., Baker, D.C., TraubDargatz, J.L. 2003. A comparison of dobutamine infusion to exercise as a cardiac stress test in healthy horses. Journal of Veterinary Internal Medicine, 17:58-64. https://doi. org/10.1111/j.1939-1676.2003.tb01324.x

Gehlen, H., Marnette, S., Rohn, K., Stadler, P. 2006. Stress echocardiography in wormblood horses: comparison of dobutamine/atropine with treadmill exercise as cardiac stressors. Journal of Veterinary Internal Medicine, 20:562-568. https://doi.org/10.1111/j.1939-1676.2006. tb02897.x

Hori, Y., Sato, S., Hoshi, F., Huguchi, S. 2007. Assessment of longitudinal tissue Doppler imaging of the left ventricular septum and free wall as an indicator of left ventricular systolic function in dogs. American Journal of Veterinary Research, 68(10):1051-1057. https://doi.org/10.2460/ajvr.68.10.1051

Kamaran, M., Teague, S.M., Finkelhar, R.S., Dawson, N., Bahler, R.C. 1995. Prognostic value of dobutamine stress echocardiography in patients referred because of suspected coronary artery disease. American Journal of Cardiology, 76:887-891. DOI: https://doi. org/10.1016/S0002-9149(99)80255-0

Katritsis, D.G., Karabinos, I., Papdopulos, A., Simeonidis, P., Korovesis, S., Giazityoglou, E., Karvouni, E., Voridis, E. 2005. Sustained ventricular tachycardia induced by dobutamine stress echocardiography: a prospective study. Europace, 7:433-439. https://doi. org/10.1016/j.eupc.2005.05.009

Liang, C., Yi, J.M., Sherman, L.G., Black, J., Gavras, H., Hood, W.B. 1981. Dobutamine infusion in conscious dogs with and without acute myocardial infarction: Effects on systemic hemodynamics, myocardial blood flow, and infarct size. Circulation Research, 49:170-180.

Lima, R.deS.L., DeLorenzo, A., Issa, A. 2008. Reduced adverse effects with an accelerated dobutamine stress protcol compared wiith the conventional protocol: a prospective, randomiyed, myocardial perfusion scintigraphy study. International Journal of Cardiovascular Imaging, 24:55-59.

Lu, D., Greenberg, M., Little, R., Malik, Q., Fernicola, D.J., Weissman, N.J. 2001. Accelerated Dobutamine stress testing: safety and feasibility in patients with known or suspected coronary artery disease. Clinical Cardiology, 24:141-145. https://doi.org/10.1002/ clc.4960240208

McEntee, K., Amory, H., Pypendop, B., Balligand, M., Clercx, C., Michaux, C., Jacqmot, O., Robert, F., Gerard, P., Pochet, T., Henroteaux, M. 1998. Effects of dobutamine on isovolumic and ejection phase indices of cardiac contractility in conscious healthy dogs. Research in Veterinary Science, 64:45-50. https://doi.org/10.1016/S0034-5288(98)90114-X

McEntee, K., Clercx, C., Flandre, T., Jonvill, E., Pynnaert, C., van Benden, R., Ketelslegers, J.M., Michaux, C., Balligand, M., Miserque, N., Henroteaux, M. 2000. Hormonal response to dobutamine cardiac stress testing in a conscious canine model of early left ventricular dysfunction induced by chronic rate overload. Journal of Veterinary Cardiology, 2:7-14. https://doi.org/10.1016/S1760-2734(06)70006-6

McEntee, K., Clercx, C., Pypendop, B., Peeters, D., Balligand, M. 1996. Cardiac performance in conscious healty dogs during dobutamine infusion. Research in Veterinary Science, 61:234- 239. https://doi.org/10.1016/S0034-5288(96)90070-3

McEntee, K., Clercx, C., Soyeur, D., Amory, H., Michaux, C., Flandre, T., Jonville, E., Pynnaert, C., Miserque, N., Henroteaux, M. 2001. Usefulness of dobutamine stress tests for detection of cardiac abnormalities in dogs with experimentally induced early left ventricular dysfunction. American Journal of Veterinary Research, 62:448-455. https:// doi.org/10.2460/ajvr.2001.62.448

Mertes, H., Sawada, S.G., Ryan, T., Segar, D.S., Kovacs, R., Folty, J., Feigenbaum, H. 1993. Symptoms, adverse effects and complications associated with dobutamine stress echocardiography. Experience in 1118 patients. Circulation, 88:15-19.

Minors, S.L., O’Grady, M.R. 1998. Resting and dobutamine stress echocardiography factors associated with the development of occult dilated cardiomyopathy in healthy Doberman Pincher dogs. Journal of Veterinary Internal Medicine, 12:369-380.

Sahn, D.J., DeMaria, A., Kisslo, J., Weyman, A. 1978. Recommendations regarding quantitation in M-mode echocardiography: results of a survey of echocardiographic measurements. Circulation, 58:1072-1083. San Roman, J.A., Serrador, A., Ortega, J.R., Medina, A., Fernandez-Aviles, F. 2003. Diagnostic accuracy of a new shorter dobutamine infusion protocol in stress echocardiography. Heart, 89:1089-1090. http://dx.doi.org/10.1136/heart.89.9.1089

Sandersen, C.F., Detilleux, J., deMoffarts, B., VanLoon, G., Amory, H. 2006. Effect of atropinedobutamine stress test on left ventricular echocardiographic parameters in untrained warmblood horses. Journal of Veterinary Internal Medicine, 20:575-580. https://doi. org/10.1111/j.1939-1676.2006.tb02899.x

Schinkel, A.F.L., Vourvouri, E.C., Bax, J.J., Boomsma, F., Bountioukos, M., Rizzello, V., Biagini, E., Agricola, E., Elhendy, A., Roelandt, J.R.T.C., Poldermans, D. 2004. Relation between left ventricular contractile reserve during low dose dobutamine echocardiography and plasma concentrations of natriuretic peptides. Heart, 90:293-296. http://dx.doi.org/10.1136/ hrt.2002.003822

Sousa, M.G., Carareto, R., Pereira-Neto, G.B., Gerardi, D.G., Camacho, A.A. 2004. Usefulness of dobutamine stress test in the evaluation of systolic function of dogs with doxorubicin induced cardiomyopathy. Proceedings of 29th WSAVA, Free Communications, Rhodes, Greece, 6-8 October 2004, http://www.vin.com/proceedings/Proceedings. plx?CID=WSAVA2004&0=Generic

Sousa, M.G., Pereira-Neto, G.B., Carareto, R., Gerardi, D.G., Camacho, A.A. 2005 Assessment of eletrocardiographic parameters in healthy dogs undergoing dobutamine stress testing. Schweizer Archive Fur Tierheilkunde, 147:541-545. https://doi.org/10.1024/0036- 7281.147.12.541.

Spasojevic Kosic, Lj., Trailovic, D.R., Matunovic, R. 2012. Resting and dobutamine stress test induced serum concentration of brain natriuretic peptide in German Shepherd dogs. Research in Veterinary Science, 93:1446-1453. https://doi.org/10.1016/j.rvsc.2012.04.002

Suzuki, R., Matsumoto, T., Teshima, T., Mochizuki, Y., Kozama, H. 2014. Dobutamine stress echocardiography for assessment of systolic function in dogs with experimentally induced mitral regurgitation. Journal of Veterinary Internal Medicine, 28:386-392. https://doi. org/10.1111/jvim.12293

Tsutsui, J.M., Lario, F.C., Fernandes, D.R., Kowatsch, I., Sbano, J.C., Franchini Ramires, J.A., Mathias, Jr.W. 2005. Safety and cardiac chronotropic responsivness to the early injection of atropine during dobutamine stress echocardiography in the elderly. Heart, 91:1563-1567. http://dx.doi.org/10.1136/hrt.2004.054445

Wackers, F.J.T. 1993. Which pharmacological stress is optimal, Editorial comment. Circulation, 87:646-648.

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