Oral amebiosis caused by Entamoeba gingivalis, mimicking canine chronic ulcerative stomatitis in a four-year-old Basenji dog
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Abstract
Protozoan diseases within the oral cavity of companion animals are quite rare, and such cases are not common in veterinary cytology. In this case report, we describe a case of oral cavity amebiasis in a four-year-old Basenji dog with signs of severe generalized stomatitis and bilateral mandibular lymph node enlargement. In touch imprint cytologic samples, among inflammatory and epithelial cells, protozoa with morphologic features characteristic of Entamoeba gingivalis were found. After diagnosis the patient was successfully treated with a combination of local and systemic metronidazole two times per day for three weeks. Therefore, in cases of generalized gingivostomatitis in dogs, there is reason to suspect the presence of protozoal agents and pay more attention to evaluating touch imprint smears from the oral cavity.
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References
Anderson, J. G., Peralta, S., Kol, A., Kass, P. H. and Murphy, B. 2017. Clinical and Histopathologic Characterization of Canine Chronic Ulcerative Stomatitis. Veterinary Pathology, 54(3):511–519. https://doi.org/10.1177/0300985816688754.
Anderson, J.G, Kol, A., Bizikova, P., Stapelton, B.P., Ford, K., Villarreal, A., Jimenez, R.J., Vasilatis, D., Murphy, B.G. 2020. Immunopathogenesis of canine chronic ulcerative stomatitis. PLoS ONE 15(1):e0227386. https://doi.org/10.1371/journal.pone.0227386.
Ford, K.R, Anderson, J.G, Stapleton, B.L, Murhy, B.G., Santosh Kumar, T.K., Archer, T., Mackin, A. J., Wills, R. W. 2023. Medical Management of Canine Chronic Ulcerative Stomatitis Using Cyclosporine and Metronidazole. Journal of Veterinary Dentistry. 40(2):109-124. doi:10.1177/08987564221148755.
Ruparell, A., Inui, T., Staunton, R., Wallis, C., Deusch, O. and Holcombe, L.J. 2020. The canine oral microbiome: variation in bacterial populations across different niches. BMC Microbiology:20, 42. https://doi.org/10.1186/s12866-020-1704-3.
Santibáñez, R.; Rodríguez-Salas, C.; Flores-Yáñez, C.; Garrido, D.; Thomson, P. 2021. Assessment of Changes in the Oral Microbiome That Occur in Dogs with Periodontal Disease. Veterinary Science:8, 291. https://doi.org/10.3390/vetsci8120291.
Kačírová, J., Maďar, M., Štrkolcová, G., Maďari, A. and Nemcová, R. 2020. Dental Biofilm as Etiological Agent of Canine Periodontal Disease. IntechOpen. doi: 10.5772/intechopen.88305.
Bonner, M., Fresno, M., Gironès, N., Guillén, N. and Santi-Rocca, J. 2018. Reassessing the Role of Entamoeba gingivalis in Periodontitis. Frontiers in Cellular and Infection Microbiology, 8:379. doi:10.3389/fcimb.2018.00379.
Bao X., Wiehe R., Dommisch H., Schaefer A.S. 2020. Entamoeba gingivalis Causes Oral Inflammation and Tissue Destruction. Journal of Dental Research, 99(5):561-567. doi:10.1177/0022034520901738.
Jiao, J., Bie, M., Xu, X., Duan, D., Li, Y., Wu, Y. and Zhao, L. 2022. Entamoeba gingivalis is associated with periodontal conditions in Chinese young patients: A cross-sectional study. Frontiers in Cellular and Infection Microbiology, 12:1020730. doi: 10.3389/fcimb.2022.1020730.
Yaseen, A., Mahafzah, A., Dababseh, D., Taim, D., Hamdan, A.A., Al-Fraihat, E., Hassona, Y., Sahin, G.Ö., Santi-Rocca, J. and Sallam, M. 2021. Oral Colonization by Entamoeba gingivalis and Trichomonas tenax: A PCR-Based Study in Health, Gingivitis, and Periodontitis. Frontiers in Cellular and Infection Microbiology, 11:782805. doi: 10.3389/fcimb.2021.782805.
Fadhil Ali Malaa, S., Abd Ali Abd Aun Jwad, B., & Khalis Al-Masoudi, H. 2022. Assessment of Entamoeba gingivalis and Trichomonas tenax in Patients with Chronic Diseases and its Correlation with Some Risk Factors. Archives of Razi Institute, 77(1):87–93. https://doi.org/10.22092/ARI.2021.356549.1868.
Becker C., Adam A., Dommisch H., Stach, T. and Schaefer, A.S. 2023. In vitro induction of Entamoeba gingivalis cyst-like structures from trophozoites in response to antibiotic treatment. Frontiers in Cellular and Infection Microbiology, 13:1201394. doi: 10.3389/fcimb.2023.1201394.Miranda, B.P.; Miglionico, M.T.d.S.; dos Reis, R.B.; Ascenção, J.d.C.; Santos, H.L.C. 2025. Beyond Bacteria: The Impact of Protozoa on Periodontal Health. Microorganisms, 13:846. https://doi.org/10.3390/microorganisms13040846.
Patel, N., Colyer, A., Harris, S., Holcombe, L., & Andrew, P. 2017. The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease. The Journal of Eukaryotic Microbiology, 64(3):286–292. https://doi.org/10.1111/jeu.12359.
Randolph, N.K., McAloney, C.A., Ossiboff, R., Hernandez, B., Cook, M. and Hokamp, J.A. 2022. Nasal colonization by Entamoeba gingivalis in a 13-year-old Italian Greyhound. Veterinary Clinical Pathology, 51:269–272. doi: 10.1111/vcp.13064.
Simitch, T. 1938. Sur une amibe buccale du chien Entamœba canibuccalis n. sp. Annales de Parasitologie Humaine et Comparée, 16:251-253.