Hematological, biochemical, and acid-base response of trotters to submaximal exercise at the end of the horse racing season
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Abstract
Depending on intensity, physical exercise in horses causes various changes in the parameters of hematological, biochemical, acid-base, and electrolyte status, which can affect the health and athletic performance of the horse. This study’s objective was to look at how submaximal exercise at the end of the racing season affected the horses’ hematological, biochemical, acid-base, and electrolyte status markers.
In this study, eight (n=8) trotters, aged 4±2 years, were involved. Venous blood samples were drawn from each horse by jugular puncture before and after exercise to determine hematologic, biochemical, acid-base and electrolyte parameters. The submaximal physical exercise in this study was two intervals of 2,000 m of slow trotting and two consecutive runs of 500 m at submaximal level.
Hematocrit (HCT), red blood cell (RBC) and monocyte count, hemoglobin (HGB) concentration, aspartate aminotransferase (AST) activity, and glucose concentration increased significantly after the exercise. Additionally, significant decreases in venous blood pH, bicarbonate (HCO3-) and total CO2 (TCO2) concentration, base excess of the extracellular fluid (BEecf), and ionized Ca2+ (iCa2+) concentrations were established after exercise. In contrast, partial pressure of CO2 (pCO2), total concentration of weak acids (Atot), the anion gap (AG), and total protein and lactate concentrations were significantly higher after exercise. Considering the significant changes in the parameters of hematological, biochemical, and acid-base status after submaximal exercise, determining those parameters would be useful for monitoring the health and performance of trotters.
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References
Adamu L., Adzahan N. M., Abdullah R., Ahmad B. 2012. Changes in biochemical, hematological and cytokine in endurance horses with metabolic crises. Journal of Animal and Veterinary Advances, 11:3431-3436.
Aguilera-Tejero E., Garfia B., Estepa J.C., López I., Mayer-Valor R., Rodríguez M. 1998. Effects of exercise and EDTA administration on blood ionised calcium and parathyroid hormone in horses. American Journal of Veterinary Research, 59:1605-1607.
Aguilera-Tejero E., Estepa J. C., López I., Bas S., Mayer-Valor R., Rodríguez M. 2000. Quantitative analysis of acid-base balance in show jumpers before and after exercise. Research in Veterinary Science, 68: 103-108. https://doi.org/10.1053/rvsc.1999.0341
Arfuso F., Giannetto C., Giudice E., Fazio F., Panzera F., Piccione G. 2020. Venous blood acid-base status in show jumper horses subjected to different physical exercises. Journal of Equine Veterinary Science, 94, 103251. https://doi.org/10.1016/j.jevs.2020.103251
Assenza A., Bergero D., Congiu F., Tosto F., Giannetto C., Piccione G. 2014. Evaluation of serum electrolytes and blood lactate concentration during repeated maximal exercise in horse. Journal of Equine Veterinary Science, 34:1175-1180. https://doi.org/10.1016/j.jevs.2014.07.001
Belcher C. P., Pemberton C. L. 2012. The use of the blood lactate curve to develop training intensity guidelines for the sports of track and field and cross-country. International Journal of Exercise Science, 5:148-159.
Coggan A.R. 1991. Plasma glucose metabolism during exercise in humans. Sports Medicine, 11:102-124. https://doi.org/10.2165/00007256-199111020-00003
Constable P. D. 2014. Acid-base assessment: when and how to apply the Henderson-Hasselbalch equation and strong ion difference theory. Veterinary Clinics: Food Animal Practice, 30:295-316. https://doi.org/10.1016/j.cvfa.2014.03.001
Dahl S., Cotrel C., Leleu C. 2006. Optimal active recovery intensity in Standardbreds after submaximal work. Equine Veterinary Journal, 38:102-105. https://doi.org/10.1111/j.2042-3306.2006.tb05522.x
Evans D. L. 2007. Physiology of equine performance and associated tests of function. Equine Veterinary Journal, 39:373-383. https://doi.org/10.2746/042516407x206418
Fazio F., Assenza A., Tosto F., Casella S., Piccione G., Caola G. 2011. Training and haematochemical profile in Thoroughbreds and Standardbreds: A longitudinal study. Livestock Science, 141:221-226. https://doi.org/10.1016/j.livsci.2011.06.005
Geiser D.R., Andrews F.M., Rohrbach B.W., White S.L., Maykuth P.L., Green E.M., Provenza M.K. 1995. Blood ionised calcium concentrations in horses before and after the cross-country phase of 3-day event competition. American journal of veterinary research, 56:1502-1505.
Goundasheva D., Sabev S. 2011. Influence of exercise on acid-base, blood gas and electrolyte status in horses. Trakia Journal of Sciences, 9:63-67.
Guhl A., Lindner A., Von Wittke P. 1996. Use of the relationship between blood lactate and running speed to determine the exercise intensity of horses. Veterinary Record, 139:108-110. https://doi.org/10.1136/vr.139.5.108
Hodgson D.R. 1985. Myopathies in athletic horses. The Compendium on continuing education for the practicing veterinarian (Princeton Junction, New Jersey, USA), 7:551-556.
Jagrič-Munih S., Nemec-Svete A., Zrimšek P., Kramarič P., Kos-Kadunc V., Vovk T., Kobal S. 2012. Plasma malondialdehyde, biochemical and haematological parameters in standardbred horses during a selected field exercise test. Acta Veterinaria, 62:53-65. https://doi.org/10.2298/AVB1201053J
Jovanović L., Nedić S., Bošnjaković D., Milanović S., Stojić M., Vujanac I., Prodanović R., Kirovski D. 2022. Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle. Veterinarski Glasnik, 76:25-36. https://doi.org/10.2298/VETGL210130004J
Kingston J. K. 2008. Hematologic and serum biochemical responses to exercise and training. In Equine Exercise Physiology, Edinburgh: W.B. Saunders. https://doi.org/10.1016/B978-070202857-1.50019-6
Kirsch K., Sandersen C. 2020. Traditional and quantitative analysis of acid-base and electrolyte imbalances in horses competing in cross-country competitions at 2-star to 5-star level. Journal of Veterinary Internal Medicine, 34:909-921. https://doi.org/10.1111/jvim.15708
Kupczyński R., Śpitalniak K. 2015. Analysis of acid–base balance as well as hematological and biochemical parameters in horses of combined driving discipline. Archives of Animal Breeding, 58:221-228. https://doi.org/10.5194/aab-58-221-2015
Lindinger M. I. 2014. Acid-base physiology at rest, during exercise and in response to training. In Equine Sports Medicine and Surgery. Edinburgh: W.B. Saunders.
Marlin D., Nankervis K. J. (2013). Equine exercise physiology. Blackwell Scientific Publications, Oxford, UK.
Maśko M., Domino M., Jasiński T., Witkowska-Piłaszewicz O. 2021. The physical activity-dependent hematological and biochemical changes in school horses in comparison to blood profiles in endurance and race horses. Animals, 11, 1128. https://doi.org/10.3390/ani11041128
McCutcheon L. J., Geor R. J., Hare M. J., Ecker G. L., Lindinger M. I. 1995. Sweating rate and sweat composition during exercise and recovery in ambient heat and humidity. Equine Veterinary Journal, 27:153-157. https://doi.org/10.1111/j.2042-3306.1995.tb05022.x
Oliveira C. A., Azevedo J. F., Miranda A. C. T., Souza B. G., Ramos M. T., Costa A. P. D., Baldani C. D., Silva V. P., Almeida F. Q. 2014. Hematological and blood gas parameters’ response to treadmill exercise test in eventing horses fed different protein levels. Journal of Equine Veterinary Science, 34: 1279-1285. https://doi.org/10.1016/j.jevs.2014.09.007
Ostaszewski P., Kowalska A., Szarska E., Szpota´nski P., Cywinska A. P., Bałasinska B., and Sadkowski T. 2012. Effects of ß-hydroxy-ß-methylbutyrate and oryzanol on blood biochemical markers in exercising Thoroughbred race horses. Journal of Equine Veterinary Science, 32:542–551.
Padalino B., Rubino G., Lacinio R., Petazzi F. 2014. Observations on the hematology of standardbred horses in training and racing in southern Italy. Journal of Equine Veterinary Science, 34:398-402. https://doi.org/10.1016/j.jevs.2013.07.018
Piccione G., Casella S., Giannetto C., Messina V., Monteverde V., Caola G., Guttadauro S. 2010. Haematological and haematochemical responses to training and competition in standardbred horses. Comparative clinical pathology, 19:95-101. https://doi.org/10.1007/s00580-009-0902-z
Piccione G., Casella S., Monteverde V., Giannetto C., Caola G. 2008. Haematological modifications during official 1600 and 2000 meters trot races in Standardbred horses. Acta veterinaria, 58:325-332. https://doi.org/10.2298/AVB0804325P
Rivero J. L., Piercy R. J. 2008. Muscle physiology: responses to exercise and training. In Equine exercise physiology, Edinburgh: W.B. Saunders. https://doi.org/10.1016/B978-070202857-1.50005-6
Slijepčević D., Savić R., Trailović D. 2014. The influence of physical exertion on basic hematological parameters values and heart rate in trotters. Veterinarski glasnik, 68:291-300. https://doi.org/10.2298/VETGL1406291S
Soares J. C. M., Zanella R., Bondan C., Alves L. P., de Lima M. R., da Motta A. C., Zanella E. L. 2011. Biochemical and antioxidant changes in plasma, serum, and erythrocytes of horses before and after a jumping competition. Journal of Equine Veterinary Science, 31:357-360. https://doi.org/10.1016/j.jevs.2011.03.017
Soma L.R., Uboh C.E., Nann L., Gerber A.L. 1996. Prerace venous blood acid-base values in standardbred horses. Equine Veterinary Journal, 28:390-396. https://doi.org/10.1111/j.2042-3306.1996.tb03110.x
Soma L.R., Stefonovski D., Robinson M.A., Tsang D.S., Haughan J., Boston R.C. 2022. Prerace venous blood gases and acid-base values in Standardbred horses: effects of geography, season, prerace furosemide, gender, age, and trainer using big data analytics. American Journal of Veterinary Research, 83:1-9. https://doi.org/10.2460/ajvr.22.01.0013
Soroko M., Śpitalniak-Bajerska K., Zaborski D., Poźniak B., Dudek K., Janczarek I. 2019. Exercise-induced changes in skin temperature and blood parameters in horses. Archives of Animal Breeding, 62:205-213. https://doi.org/10.5194/aab-62-205-2019
Taylor L.E., Ferante P.L., Kronfeld D.S., Meacham N.T. 1995. Acid-base variables during incremental exercise in sprint-trained horses fed a high-fat diet. Journal of Animal Science, 73:2009-2018. https://doi.org/10.2527/1995.7372009x
Vazzana I., Rizzo M., Dara S., Niutta P.P., Giudice E., Piccione G. 2014. Haematological changes following reining trials in quarter horses. Acta Scientiae Veterinariae, 42:1-5.
Viu J., Jose-Cunilleras E., Armengou L., Cesarini C., Tarancón I., Rios J., Monreal L. 2010. Acid-base imbalances during a 120 km endurance race compared by traditional and simplified strong ion difference methods. Equine Veterinary Journal, 42:76-82. https://doi.org/10.1111/j.2042-3306.2010.00213.x
Waller A. P., Lindinger M. I. 2023. Tracing acid-base variables in exercising horses: Effects of pre-loading oral electrolytes. Animals, 13, 73. https://doi.org/10.3390/ani13010073
Waller A., Heigenhauser G. J., Lindinger M. I. 2007. Electrolyte supplementation after prolonged moderate-intensity exercise results in decreased plasma [TCO2] in Standardbreds. Equine and Comparative Exercise Physiology, 4:149-158. https://doi.org/10.1017/S1478061507890086
Waller A., Lindinger M. I. 2005. Physicochemical analysis of acid–base status during recovery from high intensity exercise in Standardbred racehorses. Equine and Comparative Exercise Physiology, 2:119-127. https://doi.org/10.1079/ECP200549