Ultrastructural organization of liver hepatocytes of the anglo-nubian goat

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Anna Prusakova
Nikolay Zelenevskiy
Aleksey Prusakov
Anatoly Yashin
Vladimir Ponamarev


The aim of the study was to establish the ultrastructural organization of hepatocytes of the Anglo-Nubian goat.Livers of adult goats of the Anglo-Nubian breed served as the material for the study. The study was carried out using the electron microscopic method. For this purpose, fragments of liver parenchyma tissues, no larger than 2.0 mm3, were selected. The tissue samples were fixed in a 2.0% glutaraldehyde solution on a cacodylate buffer (pH 7.2-7.4) for 2 hours. Then, they were washed in three portions of the same buffer and post-fixed in a 1.0% solution of osmium tetrachloride (prepared in cacodylate buffer, pH 7.2-7.4) for 1 hour. Samples were dehydrated in increasing concentrations of alcohol and anhydrous acetone. The tissue fragments were then embedded in Epon-812. Ultrathin sections were obtained using an ultramicrotome (LKB-III, Sweden) and processed with 2.0% aqueous uranyl acetate and lead citrate solutions. The resulting ultrathin sections were photographed using a Jem-1011 electron microscope (JEOL, Japan) at 2500-3000× magnification.It was established that two morphological types of hepatocytes can be distinguished in the liver parenchyma of the Anglo-Nubian goat at the ultrastructural level – dark and light, both with characteristic morphological features. Dark hepatocytes have a high electron-optical density due to the presence of a larger number of organelles. Light hepatocytes are characterized by a smaller number of organelles and a lower electron-optical density than dark hepatocytes.


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Prusakova, A., Zelenevskiy , N., Prusakov, A., Yashin, A., & Ponamarev, V. (2023). Ultrastructural organization of liver hepatocytes of the anglo-nubian goat. Veterinarski Glasnik, 77(2), 176–187. https://doi.org/10.2298/VETGL230615007P
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Baryshev V. A., Popova O. S., Ponamarev V. S. 2022. New methods for detoxification of heavy metals and mycotoxins in dairy cows. Online Journal of Animal and Feed Research, 12(2): 8188. https://doi.org/10.51227/ojafr.2022.11

Bilqees F.M., Shabbir I. 2010. Histopathology of goat liver associated with Fasciola gigantica: goat liver histopathology, VDM Verlag Dr. Müller.

Braun U., Jacquat D., Steininger K. 2013. Ultrasonographic examination of the abdomen of the goat. II. Liver, spleen, urinary tract and greater omentum. Schweizer Archiv fur Tierheilkunde, 155(3):185–195. https://doi.org/10.1024/0036-7281/a000442

Braun U., Steininger K. 2011. Ultrasonographic characterization of the liver, caudal vena cava, portal vein, and gallbladder in goats. American Journal of Veterinary Research, 72(2):219–225. https://doi.org/10.2460/ajvr.72.2.219

Drozdova L.I. 2004. Histohematic barriers and their role in pathology. Omsk Scientific Bulletin, S1-1(26): 179-180.

Drozdova L.I., Kundryukova U.I. 2010. Poultry liver - a living laboratory for assessing the quality of feeding and maintenance. Agrarian Bulletin of the Urals, 5(71): 68–70.

Ghosh A., Awal A., Khan A.H.N.A., Alam G.S., Islam S., Bari A.S.M. 2014. Effects of spirulina in arsenic poisoning in the Black Bengal goat. Turkish Journal of Veterinary & Animal Sciences, 38(1): 63-72. https://doi.org/10.3906/vet-1305-10

Kuznetsov Yu.E., Lunegov A.M., Ponamarev V.S., Romashova E.B. 2022. Correlation interaction of total bile acids with basic blood biochemical indicators in minks (Mustela vison Schreber, 1777). Agricultural Biology, 57(6): 12171224 https://doi.org/10.15389/agrobiology.2022.6.1217rus

Ponamarev V., Yashin A., Prusakov A., Popova O. 2022. Influence of modern probiotics on morphological indicators of pigs’ blood in toxic dyspepsia. Agriculture Digitalization and Organic Production: Proceedings of the Second International Conference, St. Petersburg, 133142. https://doi.org/10.1007/978-981-19-7780-0_12

Prusakov A. V., Shchipakin M. V., Barteneva Yu. Yu., Virunen S. V., Bylinskaya D. S., Vasiliev D. V. 2017. Sources of blood supply to the liver of a domestic cat. Issues of Legal Regulation in Veterinary Medicine, 2: 123-125.

Prusakova, A.V., Zelenevsky N.V. (2020). Histological organization of the liver of an Anglo-Nubian goat. Hippology and Veterinary Medicine, 3(37): 158-161.

Reynolds E. S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology, 17: 208-212.

Semchenko V.V., Samuseva R.P., Moiseeva M.V. 1999. International histological nomenclature. Kolosova: Omsk.

Sethi L., Suri S., Sarma K., Sasan J.S. 2021. Histomorphology and histochemistry of liver of adult Bakerwali and non-descript goats of Jammu. Journal of Animal Research, 11(3):487-495. https://doi.org/10.30954/2277-940X.03.2021.20.

Weekly B. 1975. Electron microscopy for beginners. Mir: M.

Yashin A.V., Prusakov A.V., Kalyuzhny I.I., Kovalev S.P., Kopylov S.N., Dinisenko V.N., Radnatarov V.D., Elenschleger A.A., Kulyakov G.V. 2021. Non-infectious pathology of cattle in farms with industrial technology: textbook for free software. St. Petersburg: Lan.