Serological profile and pleurisy lesions associated with Actinobacillus pleuropneumoniae in pig farms in North Macedonia

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Branko Angjelovski
Aleksandar Janevski
Elena Atanaskova Petrov
Clara Marin Orenga
Jovan Bojkovski


Actinobacillus pleuropneumoniae (App) is one of the most important swine respiratory pathogens that causes porcine pleuropneumonia and massive financial losses in pig industry. The objective of this study was to investigate App infection in five pig herds from North Macedonia experiencing clinical respiratory infections by serological testing and a slaughterhouse pleurisy evaluation system (SPES). In total, 250 blood samples were taken from pigs aged 6, 10, 14, 18, and 22 weeks. Ten animals per age category from each farm were sampled and analyzed for presence of antibodies against App. At the slaughterhouse, 50 lungs per herd from slaughtered age pigs were scored by the SPES for the presence of lesions associated with chronic pleurisy. The overall seroprevalence to App was 65.6%, ranging from 34% to 98% at the farm level. The highest seroprevalence was found in 6-week-old pigs in all farms, while significant differences were observed between farms in the 10-, 14-, 18-, and 22-week-old pig groups. Pleurisy associated with App was found in 26.4% of all examined lungs, with the mean SPES score being 0.75 (0.14 - 1.10). The percentage of SPES scores of 0, 1, 2, 3, and 4 in all lungs were 73.4%, 1.6%, 8.8%, 8.4%, and 7.6% respectively. A significant difference in mean SPES score was obtained between two farms. High seroprevalences of App detected on the tested farms were probably due to constant natural infection. The highest seroprevalences, measured in the youngest pigs, could be due to maternally-derived antibodies. Higher seroprevalence against App and lower SPES scores in some farms suggests immunity resulting from infection by corresponding field serotypes.


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Angjelovski, B., Janevski, A., Atanaskova Petrov, E., Marin Orenga, C., & Bojkovski, J. (2023). Serological profile and pleurisy lesions associated with Actinobacillus pleuropneumoniae in pig farms in North Macedonia. Veterinarski Glasnik, 77(2), 137–148.
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Baraldi T.G., Cruz N.R.N., Pereira D.A., Galdeano J.V.B., Gatto I.R.H., Silva A.F.D., Panzardi A., Linhares D.C., Mathias L.A., de Oliveira L.G. 2019. Antibodies against Actinobacillus pleuropneumoniae, mycoplasma hyopneumoniae and influenza virus and their relationships with risk factors, clinical signs and lung lesions in pig farms with one-site production systems in Brazil. Preventive Veterinary Medicine, 171: 104748

Calsamiglia M., Pijoan C., Bosch G.J. 1999. Profiling Mycoplasma hyopneumoniae in farms using serology and a nested PCR technique. Journal of Swine and Health Production, 7(6): 263-268.

Chiers K., Donné E., Van Overbeke I., Ducatelle R., Haesebrouck F. 2002. Evaluation of serology, bacteriological isolation and polymerase chain reaction for the detection of pigs carrying Actinobacillus pleuropneumoniae in the upper respiratory tract after experimental infection. Veterinary Microbiology, 88(4): 385-392.

Desrosiers R. 2004. Epidemiology, diagnosis and control of swine diseases. In the Proceedings of 35th Annual meeting of the American Association of Swine Veterinarians, Des Moines Marriott. Des Moines, Iowa, 9-34.

Di Provvido A., Trachtman A.R., Farina E., Vaintrub M.O., Fragassi G., Vignola G., Marruchella G. 2019. Pleurisy evaluation on the parietal pleura: An alternative scoring method in slaughtered pigs. Journal of Swine and Health Production, 27(6): 312­316.

Dottori M., Nigrelli A.D., Bonilauri P., Merialdi G., Gozio S., Cominotti F. 2007. Proposta per un nuovo Sistema di punteggiatura delle pleuriti suine in sede di macellazione: la griglia SPES (Slaughterhouse Pleurisy Evaluation System). Large Animal Review, 13:161­165.

Fablet C., Marois C., Dorenlor V., Eono F., Eveno E., Jolly J.P., Le Devendec L., Kobisch M., Madec F., Rose N. 2012. Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds. Research in Veterinary Sciences, 93:627–630.

Fitzgerald R.M., O’Shea H., Manzanilla E.G., Moriarty J., McGlynn H., Calderón Díaz J.A. 2020. Associations between animal and herd management factors, serological response to three respiratory pathogens and pluck lesions in finisher pigs on farrow-to-finish farms. Porcine Health Management, 6(1): 1-10.

Fraile L., Alegre A., López­Jiménez R., Nofrarías M., Segalés J. 2010. Risk factors associated with pleuritis and cranio­ventral pulmonary consolidation in slaughter aged pigs. Veterinary Journal, 184: 326¬-333.

Galdeano J.V., Baraldi T.G., Ferraz M.E., de Souza Almeida H.M., Mechler-Dreibi M.L., Costa W.M., Montassier H.J., Mathias L.A., De Oliveira L.G. 2019. Cross-sectional study of seropositivity, lung lesions and associated risk factors of the main pathogens of Porcine Respiratory Diseases Complex (PRDC) in Goiás, Brazil. Porcine Health Management, 5(1): 1-10.

Giménez-Lirola L.G., Jiang Y.H., Sun D., Hoang H., Yoon K.J., Halbur P.G., Opriessnig T. 2014. Simultaneous detection of antibodies against Apx toxins ApxI, ApxII, ApxIII, and ApxIV in pigs with known and unknown Actinobacillus pleuropneumoniae exposure using a multiplexing liquid array platform. Clinical and Vaccine Immunology, (1): 85-95.

Gottschalk M, Broes A. 2019. Actinobacillosis. In Diseases of Swine. Eds. J.J. Zimmerman, L.A. Karriker, A. Ramirez, K..J Schwartz, G.W. Stevenson, J. Zhang, Wiley Blackwell, New Jersey, pp.749-766.

Kim B., Hur J., Lee J.Y., Choi, Y., Lee, J.H. 2016. Molecular serotyping and antimicrobial resistance profiles of Actinobacillus pleuroneumoniae isolated from pigs in South Korea. Veterinary Quarterly, 3:137-44.

Liao S.W., Lee J.J., Chen F., Lee W.C., Wu Y.C., Hsuan S.L., Kuo C.J., Chang Y.C., Chen T.H. 2017. Evaluation of lung scoring system and serological analysis of Actinobacillus pleuropneumoniae infection in pigs. Pakistan Veterinary Journal, 37(3): 340-344.

Merialdi G., Dottori M., Bonilauri P., Luppi A., Gozio S., Pozzi P., Spaggiari B., Martelli P. 2012. Survey of pleuritis and pulmonary lesions in pigs at abattoir with a focus on the extent of the condition and herd risk factors. Veterinary Journal, 193: 234 ­239.

Meyns T., Van Steelant J., Rolly E., Dewulf J., Haesebrouck F., Maes D. 2011. A cross­sectional study of risk factors associated with pulmonary lesions in pigs at slaughter. Veterinary Journal, 187: 388­392.

Pepovich R., Hristov K., Nikolov B., Genova K., Ivanova, E. 2022. Seroprevalence of Actinobacillus pleuropneumoniae infection in pigs from Bulgaria. Bulgarian Journal of Veterinary Medicine, 25(2): 333-338.

Rodrigues da Costa M., Fitzgerald R.M., Manzanilla E.G., O’Shea H., Moriarty J., McElroy M.C., Leonard F.C. 2020. A cross-sectional survey on respiratory disease in a cohort of Irish pig farms. Irish Vet Journal, 73(1): 1-10.

Sipos W., Cvjetkovic, V., Dobrokes B., Sipos, S. 2021. Evaluation of the Efficacy of a Vaccination Program against Actinobacillus pleuropneumoniae based on Lung-Scoring at Slaughter. Animals, 11, 2778.

Statistica (Data Analysis Software System). 2007. v.8.0., StatSoft, Inc., USA (

Tegetmeyer H.E., Jones S.C., Langford P.R., Baltes N. 2008. ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology, 128: 342-353.

Vigre H., Ersbøll A.K., Sørensen V. 2003. Decay of acquired colostral antibodies to Actinobacillus pleuropneumoniae in pigs. Journal of Veterinary Medicine, Series B, 50: 430–435.

Yoeger M.J, VanAlstine W.G. 2019. Respiratory System. In Diseases of Swine. Eds. J.J. Zimmerman, L.A. Karriker, A. Ramirez, K.J Schwartz, G.W. Stevenson, J. Zhang, Wiley Blackwell, New Jersey, pp. 393-407.

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