@article{Aksentijević_Ašanin_Nišavić_Marković_Milanov_Mišić_2017, place={Belgrade, Serbia}, title={Antibiotic resistance in bacteria isolated from fish in Serbia}, volume={71}, url={https://veterinarskiglasnik.rs/index.php/vg/article/view/VETGL170329005A}, DOI={10.2298/VETGL170329005A}, abstractNote={<p><em>Introduction</em>. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated.</p> <p><em>Materials and Methods</em>. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3<sup>rd</sup> and 4<sup>th </sup>generation cefalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates.</p> <p><em><em>Results and Conclusions</em>. </em>Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics<em>. </em>In<em> <em>A. hydrophila </em></em>isolated from a guppy<em> (<em>Poecilia reticulata</em>) </em>sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the<em> <em>rmtB</em> </em>gene<em>. <em>Pseudomonas</em> </em>isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3<sup>rd</sup> and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three <em><em>Pseudomonas</em> isolates from common carp </em>(<em>Cyprinus carpio</em>) were found to be resistant to colistin with MIC values of 4  μg/mL.</p>}, number={1}, journal={Veterinarski Glasnik}, author={Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan}, year={2017}, month={May}, pages={24–34} }