@article{Topić Popović_Kazazić_Čož-Rakovac_2022, place={Belgrade, Serbia}, title={MALDI-TOF MS profiling of piscine Acinetobacter species from wastewater-related waters}, volume={76}, url={https://veterinarskiglasnik.rs/index.php/vg/article/view/204}, DOI={10.2298/VETGL211201005T}, abstractNote={<p><em>Acinetobacter</em> species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied to <em>Acinetobacter</em> from fish. The aims of the study were, thus, to identify and profile the <em>Acinetobacter</em> species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI-TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDI-TOF MS, with the samples prepared by ethanol/formic acid extraction.The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were <em>A. johnsonii</em> (79.31 %), <em>A. pittii</em> (10.34 %), <em>A. tandoii</em> (3.44 %), <em>A. guilouiae</em> (3.44 %), and <em>A. gerneri</em> (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI‐TOF MS gave excellent identification and profiling results for piscine <em>Acinetobacter</em> species from the wastewater-affected waterways. It is a recommendable technique for future <em>Acinetobacter</em> species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.</p>}, number={2}, journal={Veterinarski Glasnik}, author={Topić Popović, Natalija and Kazazić, Snježana and Čož-Rakovac, Rozelindra}, year={2022}, month={Dec.}, pages={160–167} }